We use reverse micelles (RMs) as our model system to study the impact of osmolytes on water at the nanoscale. Reverse micelles are characterized by w0 = [polar component]/[surfactant]. In our experiment, we focus on 5 different w0 (w0 = 1, 5, 10, 20, 40) and 3 concentrations of urea (0.5M, 1M, 2M). The Dynamic Light Scattering (DLS) illustrates a linear relationship between reverse w0 (the ratio) and size. Additionally, the DLS data indicates that the impact of osmolytes on size for small reverse micelles is not significant. Furthermore, Red-Edge Emission Spectroscopy shows a bigger impact on emission for smaller reverse micelles compared to the larger w0 samples.
Nguyen, Tran; Levinger, Nancy E.; and Gourley, Bridget Ph.D, "Impact of Urea on Size and Red-Edge Emission Spectroscopy of Reverse Micelles" (2022). Annual Student Research Poster Session. 101.