In-vitro Stimulation of CD4+ Lymphocytes Following Renal Ischemia/Reperfusion Injury

Authors

Emma G. Kennedy, DePauw University
Jason A. Collett
David P. Basile

Document Type

Poster

Publication Date

Fall 2021

Abstract

To explore the Th-17 activation mechanism, renal CD4+ cells were isolated from post ischemic rats and stimulated in-vitro with varying conditions. These conditions focused on the effect of adenosine on IL-17 response using RT-PCR. Additionally, inhibitors of adenosine were added to test their IL-17 response in conjunction with adenosine. Specifically, an inhibitor for the Orai1 calcium channel. Furthermore, adenosine agonists were tested with to determine their effect on IL-17 response in the in-vitro stimulation plate.

Comments

Funding: IU School of Medicine

Recommended Citation

Kennedy, Emma G.; Collett, Jason A.; and Basile, David P., "In-vitro Stimulation of CD4+ Lymphocytes Following Renal Ischemia/Reperfusion Injury" (2021). Annual Student Research Poster Session. 64, Scholarly and Creative Work from DePauw University.
https://scholarship.depauw.edu/srfposters/64