Actin Depolymerization by β-Amino Alcohol in Breast Cancer Cells

Authors

An Pham '25, DePauw University
Jeffery A. Hansen, DePauw UniversityFollow
Sarah Mordan-McCombs, DePauw UniversityFollow

Files

Document Type

Poster

Publication Date

Fall 2024

Abstract

As breast cancer is heterogeneous, there is a strong need to diversify current treatment and discover more options for advanced breast cancer. Pyrrolidinyl-substituted β-amino alcohol (pyrrolidine for short) is a drug synthesized from Hansen Lab at DePauw that can kill MCF-7 breast cancer cells. Previously, we have seen MCF-7 struggling to migrate in scratch assay, and maintain proper cell shape in actin fluorescent staining. By combining both tests in one experiment, we attempted to observe how their actin structure changes during migration. In this research, we have narrowed down the mechanism of action of pyrrolidine to be actin depolymerization. This effect disrupts the formation of lamellipodia and stress fibers, which prevents MCF-7 cells from migrating and surviving.

Funding and Acknowledgements

Funding provided by the Asher Research Fund.

Recommended Citation

Pham, An '25; Hansen, Jeffery A.; and Mordan-McCombs, Sarah, "Actin Depolymerization by β-Amino Alcohol in Breast Cancer Cells" (2024). Annual Student Research Poster Session. 147.
https://scholarship.depauw.edu/srfposters/147