Effects of Mono- and Di- Saccharide Osmolytes on the Stability and Folding Dynamics of src SH3

Authors

Mahmoud Abouelkheir, DePauw University
Emily Guinn, DePauw UniversityFollow

Files

Document Type

Poster

Publication Date

Fall 10-2-2019

Abstract

Osmolytes are small molecules that stabilize protein’s native structures in living systems. In order to better understand this process, we investigate how changes in specific structural properties of osmolytes affect folding dynamics and stability of proteins. This study focuses on one common structural property that varies in quantity, linkage, and arrangement amongst some osmolytes: glucose subunits. Comparing the effects of osmolytes with varying quantities of cyclic glucose subunits, varying types of glycosidic linkages of cyclic glucose subunits, and a linear glucose analogue on the stability and folding dynamics of proteins may reveal the potential role of these structures on osmolyte-protein interactions. We characterize the effects of mono- and di-saccharide osmolytes on the stability and folding kinetics of the srcSH3 model protein using sorbitol, glucose, and maltose solutions with chemical denaturant.

Funding and Acknowledgements

Funding: Science Research Fellows Program

Recommended Citation

Abouelkheir, Mahmoud and Emily Guinn. "Effects of Mono- and Di- Saccharide Osmolytes on the Stability and Folding Dynamics of src SH3." Poster presented at the DePauw University Science Research Fellows Poster Session, Greencastle, IN, October 2019.